The Whitaker Lab within the Department of Anesthesiology & Critical Care Medicine is seeking a full-time **_Research Specialist II_** to join a multidisciplinary group exploring anesthesia, blood pressure, and organ perfusion in both a rat model and a pediatric swine model. The overall goal of this research is to determine the vascular determinants of anesthesia-induced hypotension and to identify organ perfusion-dependent definitions of hypotension in the setting of general anesthesia. Our group uses cutting-edge, clinically relevant in vivo and in vitro techniques to evaluate the complex interaction of anesthetic agents, vascular physiology, blood pressure, and organ perfusion to establish best practices for intraoperative management in vulnerable patients. This research is part of an NIH NIGMS-funded R35 program aimed at studying the above in animals at the extremes of age. The lab is also interested in perinatal stroke, and a series of experiments are being performed in a piglet model to understand its pathogenesis and treatment options better. The successful applicant will be involved in both research programs. **Specific Duties & Responsibilities** + Requirements include, but are not limited to (per PI’s discretion) _Personal Qualifications_ + Excellent communication skills and ability to work within a multidisciplinary team. + Cooperative team spirit. + Ability to make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction; considers resources and lab priorities when making decisions. _Animal Care/Experimentation_ + Anesthesia, endotracheal intubation and surgery/microsurgery, including vascular cannulation, craniotomy, middle cerebral artery occlusion. + Intraoperative physiologic monitoring and arterial blood gas management. + Administration of intravenous and intraperitoneal medications. + Postoperative care. + Animal feeding & administration of medications. + Cage clean-up and sanitation. + Neurobehavioral testing. _Research Support_ + Preparation of brain fixatives and buffers. + Performance of animal perfusions & precise dissection of brain and other organs. + Preparation of sterile surgical packets. + Histologic paraffin processing to cut brain specimens. + Histologic staining. + Performance of routine essays, such as ELISA and western blotting. + Data entry, analysis, and figure preparation (preferably in Graph pad Prism) _Administrative Responsibilities_ + Maintenance of project-specific documentation (e.g., safety documentation, etc) + Maintenance of lab inventory, ordering supplies, general lab organization + Maintenance of animal and laboratory records. + Assist with ACUC protocol preparation and maintenance. _Teaching_ + Teach investigators, residents, postdocs, and other staff to perform surgical and technical procedures. + Train personnel in routine animal care, daily maintenance of animals. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + Two years laboratory experience performing relevant techniques. + Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Certified veterinary technician preferred. + Four years related laboratory work experience preferred. + Knowledge of current regulations, policies and principles for the care and use of laboratory animals. + Knowledge and experience with basic surgical and microsurgical techniques and procedures. + Ability to handle, restrain and determine the sex of laboratory animals without causing injury or harm to animal, self, and others. + Ability to understand and follow detailed oral and written instructions. + Ability to maintain records, read instructions, labels, etc. Classified Title: Research Specialist II Job Posting Title (Working Title): Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $40,900 - $71,600 Annually ($56,250 targeted; Commensurate with experience) Employee group: Full Time Schedule: Mon-Fri Exempt Status: Exempt Location: School of Medicine - East Baltimore Campus Department name: 10002728-SOM Ane Pediatric Anesthesiology Personnel area: School of Medicine Equal Opportunity Employer: Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.